Chapter 740: Take it alone (one/three)

At 12:15 in the middle of the night,
One hour before takeoff, Xu Mang was sitting in the VIP lounge with a trace of anxiety between his eyebrows. He received a call from Academician Zhong before and was told that his AI program had a very serious error, experimental data and AI data. There are several differences.
"its not right..."
"Why is this?" Xu Mang looked at the content on the laptop screen with a very confused expression. The data on the two sides could not be matched. This is very strange. Normally, this is impossible. He has never encountered it. .
Could it be...
Are you going to capsize in the gutter?
Faced with this kind of thing that might happen, Xu Mang’s self-esteem is unacceptable. He has been used to success, but suddenly faced with failure, let alone... I don’t know how to deal with it, panic? At a loss? confused?
In short... At this moment, Xu Mang has mixed feelings in his heart, which is unspeakable.
If there is a problem with one piece of experimental data, it is fine. All seven pieces of data have problems. This is very indicative. It must be a mistake. The Xuman-2 artificial intelligence system seems to be tragedy, not overthrowing. It is to develop the number three.
"Professor Xu?"
"Your coffee... pay attention to the hot mouth." A beautiful and generous flight attendant brought a cup of coffee for Xu Mang. In fact, this flight attendant shouldn't serve Xu Mang, but the boss of Magic City personally called Magic City. The chairman of the International Airport must take good care of Xu Mang, and only then did a flight attendant serve Xu Mang alone.
"Thank you."
Xu Mang replied absentmindedly, looking at the data on the screen to himself, completely turning a blind eye to the stewardess beside him. At this moment, the mobile phone on the side rang and the caller was Yang Xiaoman.
"Hey?"
"You're not going to sleep at this late?" Xu Mang answered the phone and asked curiously: "Too late to sleep is prone to wrinkles. She was originally a beautiful girl, but after a few days she will become a yellow face woman, although I seek spiritual Beautiful, but only if the other person’s appearance is up to standard."
"..."
"Stupid...I'm worried about you, and I can't sleep a little bit." Yang Xiaoman said glutinously, "What kind of trouble did I have in the end? This is about to celebrate the Chinese New Year, and I want you to go to the capital... Being considerate of others, originally having a happy New Year, now adds a lot of blockage."
Faced with Xiaoman’s constant complaints, Xu Mang just smiled and said calmly: "This is the norm... Actually I am already very free. Many scientific researchers who you don’t know or know, they are more Hard work...willing to be incognito to bury famous people and achieve earth-shaking things."
"Little Man..."
"This year's New Year... I may not be able to come back. Don't forget to come to my house on New Year's Eve. My parents miss you very much." Xu Mang said helplessly, "I have encountered a problem that cannot be solved temporarily."
Yang Xiaoman was silent for a long time and said questioningly: "I know... Don't worry about the family affairs, I will take care of it all."
after that,
The two whispered some whispers and didn't hang up until fifteen minutes before takeoff.
...
Got off the plane,
Xu Mang saw the long-awaited staff.
"Thank you."
"Waiting for me in a cold day." Xu Mang smiled bitterly: "Speaking of which there are still people in the institute? There should be no one at four in the morning, right?"
"Ok!"
"Professor Xu, I will take you to the guest house first?" the staff member asked.
Xu Mang thought for a while and said seriously: "You can send me the research institute first, I can't sleep for the time being, right... the guard should have the key to the laboratory?"
"..."
"Professor Xu, why don't you go back to the guest house first, Academician Zhong asked me to send you to the guest house." The staff member smiled bitterly: "You just got off the plane, so let's take a rest. Tomorrow... tomorrow you may be a little bit busy."
"Oh..."
soon,
Xu Mang went to the so-called guest house, which is actually the place where he visited last time. It may be the reason for the upcoming Chinese New Year. Xu Mang moved in soon and came to his room without sleep. He took it out of his handbag. After the notebook, continue to look at the data.
According to reason,
Even if it is not the upgraded CRISPR-Cas9 system, it should have a higher target accuracy, but why is the error rate of the upgraded CRISPR-Cas9 system 100%?
"Ugh..."
"I'm dying!" Xu Mang was lying on the bed, looking at the ceiling above his head. The emotions that he couldn't let go of for a while, gradually came to his mind. He really didn't have a clue. He originally thought he won, but he never thought. This just opened Pandora.
Unconsciously,
Xu Mang went into his sleep and was awakened by the phone four hours later.
"Hey?"
"Um...I'll be here right away." Xu Mang hit Hatch, rinsed away, and then went to the Institute of Biochemistry and Cell Biology of the Academy of Sciences.
quickly,
Xu Mang met Academician Zhong, the head of the Institute of Biochemistry and Cell Biology of the Academy of Sciences, but his condition was very poor, with faint bloodshot eyes in his eyes. It seemed that he didn't sleep well yesterday, so think about it... File things, how can you sleep well.
"Professor Xu Mang."
"I finally waited for you." Academician Zhong smiled bitterly: "This gap is too big. Almost your AI calculation results are wrong, and it is a very serious off-target error, Professor Xu Mang...what the is this? Happening?"
Xu Mang shook his head and said helplessly, "I don't know, so I hurried over to find out the situation."
At this time,
Academician Chu came in, and was taken aback when he saw Xu Mang, and hurriedly asked, "Professor Xu Mang?"
"This is Old Chu, Academician Chu." Academician Zhong introduced.
"Hello, Academician Chu."
After a simple greeting, the three began to talk about the root cause of the problem, but after ten minutes, they were still clueless, but the most embarrassing one was Xu Mang, after all, the problem was all on his body.
Then Xu Mang came to the laboratory, got a more detailed report, and then applied to Academician Zhong for a temporary office.
"Professor Xu Mang?"
"Have you found any problems?" Academician Zhong asked.
"Not for the time being." Xu Mang still shook his head, and said helplessly: "But academician Zhong rest assured...Since I promised to help you upgrade the CRISPR-Cas9 system, I will definitely do it, right... Can't you give me a key to this office?"
"Uh?"
"Professor Xu Mang?"
"Aren't you going back?" Academician Zhong asked.
"Ok!"
"My self-esteem won't let me go back." Xu Mang said seriously: "This matter can't be done, and I feel uncomfortable all over."
For a while,
Academician Zhong didn’t know what to say. He had listened to his old friend before, Xu Mang’s second grandfather talked about that this guy’s head is not an ordinary iron, and his temper is stubborn and ten cows can’t be pulled back. He thought it might be mixed in. With some moisture, who would have thought... is true.
"Professor Xu Mang."
"Seeing that the New Year is about to come, or do it again after the new year." Academician Zhong said.
"Forget it..."
"I also lock myself up at home. Rather than go back with troubles, it's better to be here." Xu Mang smiled bitterly: "It's not as good as the others, but the sadness is not as good as the sadness, Academician Zhong...you Talk to the researchers in the institute, come back during the Chinese New Year...I will give them a satisfactory answer and let them relax."
Hearing Xu Mang's words,
Academician Zhong knew that he was planning to resist all the responsibilities alone.
...
There are still three days before the New Year,
The Institute of Biochemistry and Cell Biology of the Academy of Sciences is empty. Almost all of them go home except for those on duty.
In the past few days,
Xu Mang was not idle. He found the problem. The upgraded CRISPR system should not be edited by Cas9 nuclease. It needs a smaller nuclease to cut 1 DNA strand. Reversal enzymes perform the task of gene editing at the cut point under the guidance of the RNA strand.
at this time,
For Xu Mang to find a brand new gene scissors, a brand new RNA-guided nuclease needs to have three characteristics, which are smaller, more stable, and more complete.
Smaller means easier to use in clinical gene therapy that requires in vivo delivery, more stable because it can maintain high enzyme activity in a wide temperature range and pH range, and safer means less off-target effects.
but...
This is not so difficult!
in fact,
Xu Mang has two new RNA-guided nucleases, namely Cpf1 and C2c1. These two proteins are smaller than Cas9 and easier to enter tissues and cells, especially Cpf1, which is very close to the recognition site when it is cut. Far, so that researchers have more choices in the choice of editing position.
But Xu Mang is more inclined to C2c1, an RNA-guided nuclease, which is smaller than Cpf1 and is easier to achieve cell-to-cell delivery through viral vectors. It is just that C2c1 has thermophilic characteristics and is difficult to develop into a useful gene editing tool. . UU reading www. uukanshu.com
No way,
C2c1 comes from acidophilic heat-resistant bacteria and requires a certain temperature to operate normally. According to a foreign research report, its optimal temperature is forty-eight degrees Celsius, which can burn ordinary mammals directly to high fever.
Redesigning the C2c1 temperature is a challenge for Xu Mang. As long as this challenge is completed, all the following problems will be solved.
At this time,
The phone rang,
The caller is the uncle.
"Hey?"
"Uncle..." Xu Mang said bitterly, "Yes...I'm in the capital, and I won't go back for the New Year, oh...I will take care of myself, don't worry... um!"
hang up,
Xu Mang continued to pounce on the process of solving the problem, but only ten minutes later, the phone rang again, this time Xiaoman called.
"Hey?"
"My husband... I bought five catties of superb tea for my dad, and bought a necklace worth more than three million yuan for my mom. It should be almost the same?" Yang Xiaoman asked, "For your relatives and friends, do you want it? Pick it up?"
"Uh..."
"Buy some gifts for the younger brothers and sisters." Xu Mang said calmly: "For example, "5 years of college entrance examination and 3 years simulation", "5 years of high school entrance examination and 3 years simulation", "the college entrance examination must be scanned"... let these Don’t want to live a good year, the little ones."
"..."
"Why are you so bad?" Yang Xiaoman said irritably, "I am also the sister of these people anyway, forget it... I won't tell you, work hard, and I will go shopping."
Snapped,
Hung up,
Xu Mang put down his phone and looked at the various materials on the table, crying without tears for a while.
Special,
Labor and management are fighting with you!
......