Chapter 195: Today's consumption is all paid by Miss Han

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When experimenting in other research groups, it is customary for students from the other research group to conduct experiments, mainly to avoid possible experimental accidents.
Laboratory safety is very important. For experiments like this kind of cross-school, it's okay if there is no accident. Once an accident occurs, it will be very troublesome to deal with, and it will even affect the level of departments and schools.
Therefore, even though Xu Qiu had copied Sun Wenyan's skills, TRPL, Tier 5 10% proficiency, he still watched with Han Jiaying.
Sun Wenyan explained while performing experimental operations in front of the experimental platform.
Similar to the ordinary fluorescence spectrum PL test, the light absorption UV-vis spectrum should be tested before the transient fluorescence spectrum TRPL is tested to determine the wavelength of the excitation light.
The laser used for TRPL is a monochromatic pulse laser. Because the signal response level is in the order of a few nanometers or even 100 picoseconds, the laser must be at least a few nanometers or even 100 picoseconds. Therefore, the price is relatively expensive, and the average price per unit is 10,000 yuan. To about 100,000 yuan.
There are a total of three wavelength lasers in Hou Kaihua's laboratory, 425, 565 and 710 nanometers, which can be applied to most experimental situations.
Sun Wenyan first confirmed the use of a 710-nanometer laser based on the results of UV-vis spectroscopy, and then tested the steady-state PL to confirm the approximate intensity of the sample's fluorescence signal.
After completing the preliminary preparations, start the TRPL test.
Sun Wenyan put the sample in the card slot in the TRPL test chamber. This card slot is a piece of up and down and a long strip, which can fix the sample on it.
This is why they asked Xu Qiu to prepare a substrate of 1 cm by 4 cm.
Subsequently, Sun Wenyan closed the sample chamber, turned on the laser, turned on the detector, operated the computer software, and started dimming.
"The signal detector is very sensitive. The number of photons passing through it in a unit time cannot be too much. The indication here is the percentage of the intensity. Generally, it is kept at about 2-4% before the test. The test effect is the best. Use a dimming mirror with a relatively high dimming magnification, and then slowly reduce the dimming magnification so that the intensity value is between 2%-4%." Sun Wenyan introduced.
Xu Qiu is clear that more than 4% should cause damage to the detector, and less than 2%, the sample signal strength is too low, the signal-to-noise ratio is low, and the data is not easy to process.
"Is the ND mirror similar to the ND mirror on the camera?" Han Jiaying asked curiously. She was more interested in SLRs before and bought a whole set of equipment, but she left them in the corner after playing with them for a few months. Gray.
"Yes, the same thing is used, and the scientific name should be called the middle gray density mirror." Sun Wenyan nodded and continued:
"The current signal intensity is 0.32%. We only need to remove a 10x ND lens. Although the ND lens we use is similar to the ND lens, the method of expressing the ND magnification is different.
In the ND mirror, ND2 means dimming 50%, ND4 means dimming 75%, and ND8 is 87.5%. The conversion is more troublesome. Ours is directly divided into dimming 2 times, 10 times, 100 times, and 1000 times. It is more convenient to test. "
After speaking, after Sun Wenyan pressed a few keys, he opened the sample compartment, removed a dimmer, then closed the sample compartment, and pressed a few more keys. The whole process was very skilled.
Han Jiaying was dazzled and puzzled, only to notice the intensity of the sample signal on the screen, which is now between 3.00%-3.20%, constantly fluctuating.
Xu Qiu understood that Sun Wenyan’s sequence of operations was to turn off the detector, turn off the laser, turn on the sample chamber, remove the dimming mirror, turn off the sample chamber, turn on the laser, and turn on the detector.
‘Turn off the detector because natural light and the intensity of the light are enough to damage the detector. ’
‘Turn off the laser because you are afraid that the laser will damage the eyes of the experimenters. Of course, the power of this laser is not enough to cause such a result, but the rules for optical experiments are like this. ’
‘This kind of precision optical instrument is troublesome to test, and the degree of automation is not high. When testing PL in the laboratory, put the sample in, set the parameters, click start, and you can wait for the results to come out. Xu Qiu complained inwardly for a while and continued to watch the experiment.
There are not many parameters that can be set in the TRPL test software. Most of them can use the default parameters without modification. There is only one more important parameter, which is the time scale.
Sun Wenyan didn't know the nature of the sample, so he chose a conservative parameter, 100 nanoseconds, which is suitable for most test situations.
Set the parameters and start the test.
The instrument started beeping "dangdang", and real-time images were displayed on the computer screen.
Each data point moves upward from the position of the horizontal axis to the positive direction of the vertical axis.
After more than two minutes, the image stopped changing, and the maximum signal strength was 10,000.
The resulting image is not a curve, but a scatter plot with a bunch of data points.
Because the data points are very many and dense, the trend can be clearly seen. From the origin, the signal strength rapidly increases to 10,000, and then slowly decreases to about 100.
Seeing the results of the experiment, Sun Wenyan said with a smile: "The measurement is fairly smooth. The baseline signal is around 100 and the signal-to-noise ratio is not high. This 100-nanometer data can be used directly. It seems that the fluorescence lifetime of your sample is not too short. "
"Well, this sample is a single component material, and several samples later are blended components. Maybe the fluorescence lifetime will be shorter. Samples No. 1 to No. 12 are similar. It should be tested under the same conditions, No. 13 to Sample No. 24 is similar, and No. 25 to No. 27 are similar." Xu Qiu nodded.
"That's great. You don't need to retest PL and UV-vis for each sample, which can save a lot of experiment time." Sun Wenyan paused and said:
"Let's add a WeChat account. You don't need to watch this experiment all the time. You can go around and call you after I finish the test to get the data. It is estimated that it will be four or five in the afternoon."
"Okay." Xu Qiu took out his mobile phone and added Sun Wenyan's WeChat.
...
After greeting teacher Hou Kaihua, the two left the laboratory and returned to the campus of Sioux City University again.
Speaking of it, Xu Qiu has been here for four years, but this is the first time he has come to the province of Jiangsu next door. Of course, it is not the kind of train that stops.
"It feels like we are traveling at public expense." Xu Qiu said with emotion.
"Yeah, I thought I would have to be busy all day long like a light source test." Han Jiaying smiled and said, "Brother, what are the next arrangements?"
"You are a landlord," Xu Qiu glanced at her, "Should I not ask you this question?"
"Yes..." Han Jiaying thought about it for a while, and said: "Let's go to dinner first, and then make plans. I'll be a guest today, and I'm not allowed to grab it."
"Well, today's consumption is all paid by Miss Han." Xu Qiu pretended to agree.
Considering that there is a test here, the two did not choose a place too far from the school. They chose the Yida Plaza around the university town. After all, it is not really a public travel. If there is a problem with the test, they may have to run again. laboratory.
Yida Plaza has a layout in major cities across the country, and investment is still biased towards large-scale chain restaurants, clothing, and department stores.
There are not too many people in the mall. It may be due to workdays. After all, except for students and teachers, most industries have now started to work again.
After shopping in the mall, Xu Qiu felt that it was not much different from the mall in Magic City.
Perhaps the prices here will be slightly lower, but it is estimated that the low will not go anywhere, at most 10%, 20% lower.
Han Jiaying stayed at the nameplate of the merchant on the first floor, staring at the name of the merchant who entered, and muttering to herself: "What to eat..."
Xu Qiu pointed to the milky white, hemispherical button under the nameplate and said, "Isn't there a button over there? You can choose one from these merchants at random. It is more suitable for people who have difficulty in choosing you."
"Then I will try." Han Jiaying pressed towards the button.
The light kept flashing behind the name of the business, and after a few seconds, it stayed on the "Chiting Teppanyaki".
"But I don't want to eat teppanyaki... one more time!"
"Kaifeng Cai... come again! This time you choose what you choose, but three things..."
"Luyu, this looks okay," Han Jiaying turned her head happily and asked, "Brother, grilled fish eat thiol?"
"Okay." Xu Qiu didn't have any comments, and made a "please" gesture toward the elevator next to him.
Lu Yu's store is on the fourth floor. It's early eleven o'clock. There are not many customers in the store and there is no need to wait.
The two sat opposite each other. Han Jiaying looked at Xu Qiu and pointed to the menu. Xu Qiu made a slight effort towards the menu.
After the silent exchange, the school girl understood, she greeted the waiter: "One portion of sea bass, sauce and pepper flavor, plus potatoes, lotus root slices, thousand-page tofu, lettuce, four vegetables, plus a cucumber, a preserved egg tofu , Two cans of Coke."
"Okay," the clerk explained patiently: "Because it is freshly baked, the waiting time for the meal may be a little longer, about 20 minutes."
"It's okay." Han Jiaying responded, and opened the public comment, looking for places to play nearby.
Xu Qiu had nothing to do. He looked up and down the opposite school girl. He hurried all the way before and didn't pay attention to the beautiful woman around him.
Today, she seems to have painted light makeup, and her upper body is a light blue hooded padded jacket. Because of the heating and air conditioning in the store, the neckline is slightly torn apart, faintly...
Han Jiaying seemed to have noticed his gaze, but still looked down at the phone, but the frequency of sliding his finger on the screen was obviously faster.
Xu Qiu was a decent person, and soon turned his attention away to observe the decoration in the store.
A few minutes later, the waiter brought two cans of canned Coke and two straws, and then he came with two small bowls and said: "Pat cucumbers, preserved eggs and tofu."
The side dishes are quite fast, but the portion is not large. It is estimated that only half a cucumber and a preserved egg were used, but the price was sold for 8 and 12 yuan.
"I have to say that the gross profit margin of the catering industry is high," Xu Qiu muttered in his heart. He heard from his fourth uncle who was a hot pot chain store before, "The probability that the gross profit margin of catering is less than 50% is a loss."
‘That’s true. Nowadays, rents are more expensive for a large-volume shopping mall, especially for the catering industry. Basically every other year or a half, the stores in the mall have to change. ’
‘Iron concrete box, merchant with flowing water. ’
After another ten minutes, Han Jiaying rushed and said: "I have made the play plan for the afternoon. After eating, we will first go to the video game city, then go to the secret room, and finally watch a movie. It just happens to be in Yida. Inside the square, there is no need to run outside."
"Um... it's okay." Xu Qiu nodded and agreed. He was a little worried about the secret room, because playing in the secret room would turn over his mobile phone. If Sun Wenyan has something to do and can't contact them, there may be problems.
But on second thought, Hou Kaihua's research team and their working hours are similar. They didn't start the experiment until 1:30 in the afternoon, and the secret room should have been over by then.
However, for the video game city, the secret room, and the movie, I always feel that adding a fast hotel afterwards will not violate any sense...
Well, I think too much, we are a serious person.
Not long after, the waiter held an iron rectangular grilled fish pan in the center of the table and introduced: "Sea bass, sauce and pepper flavor, fish can be eaten directly, and the side dishes need to be cooked before they can be eaten."
The aroma of grilled fish spreads over the surface, and it is very appetizing just by looking at it.
The fish is divided into two, and a variety of peppers, such as pickled peppers, red peppers, green peppers, and green peppers, are spread on the fish. The side dishes are under the fish, and the soup is spitting bubbles.
Xu Qiu picked up a piece of fish and took a bite.
The spines of the fish body have been removed cleanly, and the meat is very delicious and tasty.
However, it is not very spicy~EbookFREE.me~ After all, there are different eating habits in various places. Unless they are specifically labeled as Sichuan restaurants, restaurants in Jiangsu, Zhejiang and Shanghai are generally less likely to make particularly spicy dishes.
...
After eating and drinking, the two wandered to the video game city downstairs.
I happened to encounter an event. In the public comment, I could buy 25 coins for nine dollars and nine dollars. The two combined together and bought 50 coins.
Xu Qiu had two mobile phone numbers. He originally wanted to change the number and get a wave of wool, but found that this activity was bound to mobile phone information, and a mobile phone could only be used once, so he had to give up.
"It doesn't matter, just buy it if you don't have enough fun." Han Jiaying patted her chest.
Xu Qiu nodded, he almost forgot, this is a rich little woman, not bad for money.
But they now have 50 coins. As long as they don't play gambling machines such as coin pushers, under normal circumstances, it will be enough for a while.
Exchange good coins at the front desk, holding a small basket full of game coins, and the two punched cards all the way:
Shooting game, two rookies, one round trip, successfully wasted 12 coins;
For racing games, Xu Qiu’s C1 driver’s license is in hand, and I don’t think it is difficult to speak of.
In the shooting game, the two played side by side 1V1. Xu Qiu deliberately released the water, but he still won;
The dancing machine, the beautiful dance of the school girls, attracted passers-by to stop and watch;
When it was Xu Qiu's turn to play, his stiff dancing posture made him feel spicy eyes. In the end, his face blushed and he stopped dancing, and only stepped on the notes without expression.
The store is very large, but there are a lot of repeated items. The two of them strolled around. Except for the claw machine, arcade machine and various gambling machines, they almost played everything.
Finally, the two stayed in front of rows of crane machines.
At this time, Xu Qiu still had more than 20 coins in his hands.