Chapter 154: Let the world have no hard medicine

After the greeting, Zheng Lina said half-jokingly and half-seriously, and proposed to work here with Zhou Wen.
　　 Zhou Wen had a little accident.
　　 He was about to hire another employee, but he didn't expect it to be delivered.
　　 There was Tang Xin as an assistant, but it was almost enough, but he has been busy recently, and he has no time to extract high-concentration DNA.
　　 So he planned to train a successor to help him make high-concentration DNA.
　　 Tang Xin is naturally the best candidate. He knows his roots, and his personality is relatively calm, making him very suitable.
　　 Zhou Wen happily agreed to Zheng Lina's request to stay.
　　 "The specific work content, let Tang Xin teach you later."
　　 Zheng Lina was naturally overjoyed and nodded again and again, "Hmm, I know~ Thank you."
　　 Zhou Wen then said to Tang Xin: "Today I teach you to extract DNA."
　　 "Ah...I'm afraid I won't be able to learn."
　　 Tang Xin was a little surprised when he heard that. Although she had dissected mice in college, she mainly did pharmacological experiments and did not extract DNA.
　　 "Hehe, it's very simple, not as complicated as you think."
　　 Just do it, Zhou Wen immediately started teaching on-site.
　　 took a mouse out of the cage and came to the console and said, "First, kill the mouse. This is a little different from what you did for pharmacological tests. We mainly take the thigh bone marrow of the mouse..."
　　 Zhou Wen’s professor Tang Xin how to extract more mouse bone marrow.
　　 He teaches very slowly and meticulously. These experiences are derived from theory and practice after he acquires the system. It is easier to understand than any professor in the world.
　　 Tang Xin can understand it basically once.
　　 Of course, the extraction of DNA is not based on theoretical experience. Without it, it is only familiar.
　　 So after Tang Xin understood the principle, Zhou Wen directly surrendered the console and let her use it personally, while he corrected the technique on the spot nearby.
　　 includes the centrifugation time, the method of extracting the supernatant, the number of pipetting, the amount of fixed liquid added, etc., all carefully taught.
　　 And this kind of hand-in-hand teaching treatment, even Qin Haitao did not get...
　　 For the next period of time, Zhou Wen basically spent time in the Institute of Pharmaceutical Research.
　　 While teaching Tang Xin how to extract DNA, he also did pharmaceutical tasks by the way.
　　 In addition, he pumped the air conditioner to produce some optimal proportions of low-permeation liquid.
　　 is the key to ensuring that the highest concentration of DNA can be extracted every time, or else only a slightly higher concentration of DNA than the general DNA can be extracted.
　　 A week later, Tang Xin was able to complete the DNA extraction independently. Although the concentration is not high.
　　 But it is also a very remarkable thing.
　　 When the mouse DNA was observed in the electron microscope, Tang Xin was very happy.
　　 "Wow~ great..."
　　……
　　……
　　 Saturday, December 29, the day before New Year's Day.
　　 The Prion Research Institute of Jiangzhou First People's Hospital was officially established.
　　 This is also the second prion research institute in China, and its scientific research strength is recognized as the first in China.
　　The first director of the institute was Li Wenshuo.
　　 Li Wenshuo, who has been a daughter-in-law for many years, couldn't help but wet his eyes during the unveiling ceremony.
　　 He has been studying prions since the second grade, and it has been eight years now, without accomplishing anything.
　　 At the worst time, because I couldn't apply for research funding, I had to set up a joint stall with a few colleagues.
　　 Now when I think about it, it is also a bitter tear.
Because of this, Li Wenshuo is very grateful to Zhou Wen. In the darkest moment of his life, he took him a hand and arranged a chair for him on the rocket in the sky, so that he would be an unknown little figure from the domestic academic circle. He became a world-renowned prion expert!
　　 After taking photos with some leaders and scholars who came to attend the unveiling ceremony, Li Wenshuo invited several fellow scholars from home and abroad to go to the office to discuss in detail.
　　As soon as they entered the office, they found that there was a large picture of a person on the wall.
　　 "Hey, this person is so familiar."
　　 Li Wenshuo looked at the picture of Zhou Wen on the wall and smiled: "Yes, he is the inventor of PsC1, Zhou Wen, my idol."
　　 Li Wenshuo is not only grateful to Zhou Wen, he even admires this young man who is nearly 10 years younger than him. That's why he got a picture of Zhou Wen and hung it in his office, and he regarded it as a star chaser.
　　Professor Qin Ziyu, deputy director of the China Prion Research Institute, said, unfortunately, "He obviously has an outstanding talent in prions, so I don't know why he doesn't continue to study in depth?"
　　 Li Wenshuo smiled and said, "I have asked him about this question.
　　 He said that there are too many diseases in this world, and all kinds of viruses and bacteria are threatening human life and health, which often makes him feel powerless.
　　 That's why he gave up studying prions, and instead engaged in more advanced genetic research, hoping that one day he could crack the human genetic code and cure more diseases. "
　　 Qin Ziyu sighed after hearing the words: "Although I do not approve of him doing this, anyway, his courage is commendable."
　　 Li Wenshuo nodded, then smiled: "This morning, the US MIT..."
　　 2333, Science and Technology Park, Jiangzhou City, Institute of Cryptococcosis Neotype.
　　 The site renovation, equipment and personnel, etc., are all in place.
　　 The fifteen newly recruited PhDs in cell engineering also have a preliminary understanding of the projects to be studied next.
　　 And the result of understanding is that everyone is confused.
　　It is a fantasy to clone antigen serum cells based on their level;
　　 Not to mention that the content of antigenic B cells in the serum is as low as a terrible number. Even if B cells are isolated, the sensitivity and specificity of their sequence amplification and determination are very high.
　　 Even if the sequence is finally amplified and the antibody is expressed, the final effect of the antibody is still to be discussed.
　　 If they really had this level, they would not be able to find a job. They would have gone to European and American countries to earn money.
　　 When eating at noon, Xiao Yang, the head of the research group, approached Qin Haitao and asked,
Do you know what Zhousuo planned? Now the work is completely impossible to start, and there is no direction at all.

　　 Qin Haitao smiled and said: "Be safe and not irritable~ You should first understand the cryptococcus and the ingredients of the antibacterial serum. As for how to develop it, I will explain it in detail later when I wait for the week.
　　 Xiao Yang helplessly said: "Well..."
　　 Jinhu District Industrial Park, No. 404, Huanyu Institute of Biotechnology.
　　 Different from Xiao Yang, PhD students like Wang Wei have spent this time very fulfilling.
　　 According to the company's requirements, they have a detailed understanding of mainstream biotechnology at home and abroad, such as molecular biology, transgenics, bacterial communities, natural products and plant synthetic biology, clinical bioprocessing, etc.
　　 91 people, three people in a group, 30 people in a large group, learn from each other, communicate with each other, compete with each other, and initially build a good and orderly academic ecological environment.
　　 This kind of ecological academic environment is the dream of all domestic biological students.
　　 However, to build such an academic environment, the funding requirements are very high.
　　 Since this week, 210,000 yuan has been spent, which is about 3,000 yuan per person on average.
　　 You must know that this is only the most basic scientific research guarantee. If you wait for the laboratory to open and all go to the laboratory, the cost will be increased by at least 5 times.
　　 1 million a week.
　　 is 4 million a month.
　　 Don't mention anything a year, just spend at least 50 million in research funding.
　　 This is also why Zhou Wen hesitated before.
　　The task may seem simple, but the real difficulty lies in the subsequent business development.
　　 If the product cannot be developed as soon as possible, the research institute will be closed soon.
　　 At two o'clock in the afternoon, in the office of Hengfeng Building, Chen Zhiyuan looked at the thick pile of bills on his desk with a pained expression on his face.
　　 During this period of time, spending money is like running water, office space, office supplies, equipment, activity funds, etc., every day you open your eyes and you are money.
　　I don't know Chai Migui if he is not in charge. It is the first time that he knows that the boss is so stressed.
　　 The first 50 million yuan has been fully paid, but more than 3 million have been spent.
　　 This is "little money".
　　 The real big boss is purchasing equipment, and the bill on the table is the equipment list.
　　 a total of 36 million! An advance payment of 2 million has been paid.
　　Light 10 sets of Hitachi High-tech S-3700N, Hitachi High-tech S-4800, Hitachi High-tech SU-70, etc., the total price of different types of electron microscopes exceeds 20 million RMB.
　　 Horror!
　　 Chen Zhiyuan checked the bill carefully, then went to the financial room and asked them to pay the supplier the final payment.
　　 Just came out of the finance room, the phone in his pocket rang.
　　 took it out and found it was an unfamiliar local number.
　　 Chen Zhiyuan connected and said: "Hello, hello, this is Chen Zhiyuan, who are you?"
　　 "Hello, Mr. Chen, I am Meng Xiang, general manager of Jiangzhou Tianhai Intellectual Property Agency Co., Ltd., Mr. Zhou's patent application has been passed, and there are some procedures for him to come over and hand over."
　　 Chen Zhiyuan asked strangely: "What patent?"
　　 Meng Xiang: "The technological process of sildenafil pellets."
　　 "What, sildenafil?" Chen Zhiyuan exclaimed when he heard the words.
　　 Meng Xiang: "Yes! If you have time now, you might as well come to the company."
　　 Chen Zhiyuan quickly said: "Okay, OK, I'll go over now, may I ask where the address is..."
　　 Hanging up, Chen Zhiyuan almost didn't jump up with excitement.
　　 He has eaten that powerful sildenafil granule. The effect is amazing. If it is produced later, hehehe...
　　 And Zhou Wen said that this medicine will be distributed to Huanyu.
　　 Thinking of this, Chen Zhiyuan immediately left for Tianhai Company.
　　 After he arrived at the company, Zhou Wen rushed there.
　　 Then Meng Xiang's words made the two excited hearts feel like a pot of cold water was poured into them, and they were instantly cold.
　　According to the Food and Drug Administration, the new sildenafil granules are already in the category of new drugs. If you want to produce and sell, you need to go through the clinical trial process again.
　　 Zhou Wen's dream of collecting patent fees was shattered.
　　 According to the normal clinical trial process, it takes at least 3 years from human trials to market sales.
　　 Chen Zhiyuan was speechless: "It takes so long?"
　　 Meng Xiang nodded and said, "Yes! The normal process is like this~EbookFREE.me~ Zhou Wen asked: "What about the abnormal process? "
　　 Meng Xiang said with a smile: "There is another way to take the green channel of rapid review. If it works, clinical trials can be completed in about 12 months.
　　Because after all, sildenafil is also a drug that has been tested in the market for many years. On the basis of the same raw materials and formula, only the production process has been improved, and there should be room for operation.
　　You can talk to the CRO company about this specific, they should have a way. "
　　 Hearing Meng Xiang's words, Zhou Wen's eyes suddenly lit up, "Yeah, why did I forget them."
　　 The full name of the so-called CRO is called "Contract Research Organization" in Chinese.
　　 originated in the United States in the early 1980s.
　　 It is a commercial organization that provides professional services to pharmaceutical companies, medical institutions, and small and medium-sized medical device R&D companies mainly through contracts.
　　 There are three types of industrial chains.
"From the discovery of 0- (preclinical candidate compound).
　　 From-IND (Clinical Application for New Drug) verification process.
　　 INDNDA (New Drug Marketing Application) human clinical trial stage.
　　 Simply put, the purpose of CRO is to improve the efficiency of drug development.
　　You can think of all pharmaceutical companies as e-commerce stores, and CRO is the e-commerce platform.
　　 When it comes to CRO, you have to mention the domestic "Yaoming Kangde" and "Tiger Medicine".
　　 For example, Yaoming Kangde is "Ali of CRO", and their slogan is-Let the world be free of difficult medicines and difficult diseases...