Chapter 101: The Prion Research Group was formally established

　　　　 Because of the peculiarities of prions, Zhou Wen took extra care when doing experiments.
　　 Biological masks, biological gloves, and biochemical protective clothing, fully armed.
　　 And Qin Haitao and the four of them were just helping out, and they did not directly touch the utensils containing the virus.
　　 There are currently four methods for detecting prions.
　　1, electron microscope method.
　　2, capillary SDS-gel electrophoresis.
　　3, capillary electrophoresis immunoassay for fluorescently labeled peptide chains.
　　4, two-color intense fluorescent target scanning method.
　　The second type of "gel electrophoresis" is the best and most direct detection method.
　　 is mainly through physical and chemical methods-differential, ultracentrifugation, sodiumN- (lauroyl sarcosine), PK treatment, purification of PrPsc.
　　 Then perform capillary SDS-gel electrophoresis on the "capillary electrophoresis apparatus".
"According to the time and shape of the absorption peak at the wavelength of 214nm when each component passes through the detector, the prion pathogen PrPsc is identified.
　　 This method uses less specimens and does not require immunological reagents, so the judgment is intuitive.
　　The disadvantage is that it needs to use "ultracentrifuge" and "special capillary electrophoresis apparatus".
　　Ultracentrifuge is basically purchased from foreign brands, and the price is as high as RMB 500,000.
　　 The dedicated capillary electrophoresis instrument for prions is also very expensive, requiring more than 200,000 RMB.
　　 Even if it is expensive, there are few opportunities to use it.
　　 Ordinary hospitals can't even make money back for purchasing equipment, and only rich third-class hospitals like Jiangzhou Hospital will purchase them.
　　 Because of this, medical resources in big cities are scarce.
　　 The small city has a strange disease, you can't even do the test.
　　 Zhou Wen carefully prepared reagents, extracted diluents, and placed them on an ultracentrifuge to separate mutant prion proteins in accordance with physical and chemical methods.
　　 The newly upgraded biological immunology makes him even more handy.
　　 In this process, Zhou Wen is also teaching Qin Haitao and Dong Wenying, telling them what needs to be paid attention to in the process of routine prion physical and chemical tests.
　　 "When adding lauroyl, you must remember..."
　　 These experiences, let alone university professors, even the experts from the prion research institutes in China can't explain it clearly.
　　 Because the domestic research on viruses, especially prions, is basically still in its infancy, these practical experiences can only be mastered by top foreign research institutes.
　　 Such as the Yeast Prion Institute of the University of Kent, UK.
　　 For a long time, new discoveries in the field of prion proteins have been "monopolized" by several research teams in the field of yeast.
　　 Those top research institutes will not easily teach you technology.
　　 Unless you can get their offer.
　　 And Qin Haitao and the others obviously haven't realized how lucky they are. They just think Zhou Wen's explanation is very simple and easy to understand, and they even told them the essentials of the action.
　　 Qin Haitao and Dong Wenying both listened attentively when Zhou Wen was talking.
　　 As for the other two old experimenters, although they were listening on the surface, they didn't actually listen.
　　Because they feel that it is impossible to extract prions by Zhou Wen's method, this kind of teaching is nothing but paper.
　　 Zhou Wen does not have "electric eyes", how could he know these specific experimental variables, everything is just his guess.
After 　　, etc., PrPsc was purified, Zhou Wen injected the prion-containing supernatant into the Beckman P/ACE5500 for electrophoresis experiments.
　　 "Okay~" Zhou Wen breathed a sigh of relief after the injection was completed.
　　 "Qin Haitao, stay and take care of the machine. Let others wash their hands and eat."
　　 After hearing this, everyone went to the sink to wash their hands...
　　 After eating, Zhou Wen didn't dare to go anywhere, and went straight back to the laboratory.
　　The samples obtained by the conventional extraction method are too scarce, and I’m really worried if I don’t look at it myself...
　　 At half past seven in the evening, the electrophoresis experiment is over.
　　 One of the experimenters, Huang Xingzhong, took the electrophoresis analysis sheet and glanced casually, subconsciously thinking that this experiment will fail as before.
　　 However, in the next second, a look of surprise appeared on his face, "Hey, come and see, it seems that a prion has been detected..."
　　 Several other people heard the words and gathered around.
　　 "Huh, really ah."
　　 "Teacher, come and see, we have detected a prion."
　　 "Hiss... It shouldn't, how is this possible, is it a mistake..."
　　 Zhou Wen took the analysis sheet and looked at it carefully, and soon his face showed uncontrollable joy.
　　 No matter how smooth the previous experiment is, nothing will be 100% until the results come out.
　　 For example, a failure of the capillary electrophoresis instrument will also cause the test to fail.
　　 This will make all previous efforts in vain.
　　 So sometimes doing experiments, luck also accounts for part of the factor.
　　 After confirming that it was not dazzling, everyone's faces showed unspeakable excitement.
　　 To tell the truth, including Qin Haitao and Dong Wenying, do not think that prion protein can be extracted by conventional methods.
　　 Under the premise of not having any hope, it is really a proud thing to extract prion protein by conventional collection methods.
　　 After the excitement passed, Qin Haitao immediately notified Yang Liqun and Zhang Shuguang.
　　 After hearing the words, the two rushed over.
　　 "According to the test, this is an aggregate prion..."
　　 Yang Liqun and Zhang Shuguang looked at the plain electrophoresis analysis sheet, their faces were full of surprise, joy, excitement, excitement, and incredible expressions.
　　 "Is this really detected in the urine?"
　　 "It's the Zhang group! The samples were collected by me and Zhou Wen."
　　 Yang Liqun and Zhang Shuguang looked incredible when they heard Dong Wenying's words.
　　 In the case of routine testing and non-routine testing, the meaning of extracting prions is completely different.
　　 This is a very important discovery, with high research value.
　　 "You are all good. Although this is only a small step in prion research, it is a big step for China in the field of prions."
　　 "Thank you for the compliment from Team Yang. The main reason is Teacher Zhou. If he hadn't been insisting, we might have given up at first sight."
　　 Yang Liqun and Zhang Shuguang looked at Zhou Wen.
　　 Both of them have always been very optimistic about this young man, and Zhou Wen has lived up to his expectations. He has shown a very strong talent in biomedicine.
　　 and often give them some surprises.
　　 Such as this time.
　　 Yesterday they also predicted that it is absolutely impossible to extract prion protein under conventional collection methods.
　　 As a result, Zhou Wen "slapped his face" the next day.
　　 But this face is comfortable.
　　 If they can, they hope that he will continue to slap his face, it is best to open the bow left and right!
　　 After being excited, Yang Liqun and Zhang Shuguang went to the computer to analyze the sample collection records, hoping to find a certain pattern.
　　 Zhou Wen also admired Yang Liqun and Zhang Shuguang's behavior very much.
　　 This is the spirit of scientific research.
　　Although no one wants to collect prion protein in a routine state, they can't stop studying because they don't want it.
　　Only knowing what factors will cause the excretion of prion protein through body fluids can prevent and control better.
The next morning, Yang Liqun reported Zhou Wen’s findings to the hospital, requesting the official establishment of the "Prion Research Group~EbookFREE.me~ hospital leaders under the testing center. A special meeting was held to discuss whether to approve the establishment. This research group?
　　 As the First People's Hospital affiliated with Jiangzhou University, it is not too bad in terms of hardware equipment or talent pool, but why is it inferior to other hospitals?
　　 The main reason is that Jiangzhou No. 1 Hospital is well-known and loose, and there is no such thing as a
unique skill
.
　　 For example, the Jinling Military General Hospital in the province is famous throughout the country for kidney disease.
　　 For example, Huashan Hospital in Zhonghai is very impressive in dermatology, infection and neurology.
　　 Another example is Kyoto University First Hospital, which is invincible in urology.
　　 There is also the orthopedics department of Changzheng Hospital...
　　 As for the four heavenly groups, Beixiehe, Nanxiangya, Dongqilu, and Xihuaxi, they are of the immortal level, and Jiangzhou Hospital can't keep up with them.
　　 In short, Jiangzhou Hospital can be compared with other municipal hospitals in the province except Jinling, and it is not worth mentioning domestically.
　　Because of this, Jiangzhou Hospital urgently needs the same
town place
with outstanding medical skills.
　　 Finally, the research team's application was approved by a unanimous vote.
　　Because of Jiangzhou...it should be said that there are no authoritative experts on prions in the entire Sudong Province.
　　 As the initiators of the virus research, Yang Liqun, Zhang Shuguang, and Zhou Wen undoubtedly became the backbone candidates of the research team.
　　 Under the election of Yang Liqun and Zhang Shuguang, Zhou Wen became the group leader of the [Prion Research Group] of the testing center, and the two of them were honorary deputy group leaders.
　　 The first group members: Qin Haitao, Dong Wenying, Huang Xingzhong, Liu Shan...
　　.
　　
　　Ps: Sorry, please...