Chapter 16: Perfect PCR experiment

　　　　 Wu Yan knew that Zhou Wen must have a special extraction technique.
　　 But she didn't want to say it when she saw him, and she didn't force it. After all, this is other people's housekeeping skills, and it is harmful and useless to leak out.
　　 Then the two people talked about remuneration again. Wu Yan told Zhou Wen what she had said to Shi Lei again, and finally smiled and asked, "Do you have any questions?"
　　 Zhou Wen thought of the task and said, "Could you ask, am I a contract worker or a formal worker?"
　　 "Well..." Wu Yan hesitated, she didn't expect Zhou Wen to ask this question.
She believes that Zhou Wen should be very clear about the current biological situation. The contract of Jiangzhou First People’s Hospital is also so generous. Let alone a small undergraduate student, even if there are many people with master’s degree and doctoral degree in biology. It is also very desirable.
　　 He actually wants to go to the sky in one step and directly solve the establishment problem. It's really too much. She is still a contract worker.
　　 Wu Yan thought for a while and said: "Generally speaking, if you are hired by the hospital, it must be a formal worker, but you are an employment contract for the PCR team of the testing center, so I am afraid you can only be a contract worker."
　　 "Contractor..." Zhou Wen hesitated.
　　 In fact, a contract worker is still a formal worker. He really doesn't care. As long as he can go to the testing center to practice internship, he is willing even if he is a temporary worker.
　　 Isn't this a task that requires to become a formal worker within a week, he can't help but stretch a little.
　　 1000 experience points and 1000 points, there is a chance to open a black iron box.
　　 Wu Yan originally thought that the matter had been settled, but she didn't expect that there would be something wrong here, so she said painstakingly: "Although you are a contract worker, the salary is calculated on the basis of a formal job, and there is no difference.
　　 In addition, everything is done step by step. It is not too late for you to go to work and wait for a period of time to resolve the issue. "
　　 Zhou Wen asked with a smile: "You are my senior sister, and you have been in the hospital for a year. To be honest, if I really sign a contract, when will the establishment be resolved?"
　　 "Well..." Wu Yan was a little embarrassed, and she concealed her coffee cup.
　　 After putting down the cup, Wu Yan said, "Well then, I'll help you go back and ask. But to be honest, don't expect too much."
　　 Zhou Wen nodded and said with a smile: "Okay, then I will trouble Senior Sister."
　　 "You're welcome."
　　 Zhou Wen was still eccentric and didn't wait much, he got up and left after a few polite words.
　　……
　　 When he returned to the laboratory, Yang Yudong and Gu Hongyun couldn’t wait to ask him whether the interview was successful.
　　 Zhou Wen said with a smile: "I made a provision. She said she would go back to discuss with the leader, and then give me the trust after the discussion."
　　Yang Yudong said silently: "The conditions are so good, do you have to mention them?"
　　 Zhou Wen said: "I don't think it's a rare opportunity, I want to try their reserve price."
　　Yang Yudong: "……"
　　 He really wanted to use the extraction gun to get Zhou Wen's brain out a little, to see what he was thinking all day long?
　　 Zhou Wen smiled and went to do a PCR experiment.
　　The full name of PCR is PolymeraseChain.
The Chinese name of 　　 is "polymerase chain reaction", also known as "polymerase chain reaction". It is an in vitro nucleic acid amplification technology developed in the mid-1980s.
　　 The target gene or a certain DNA fragment to be studied can be amplified to 100,000 or even million times in a few hours in a test tube.
　　 Its principle is basically similar to DNA replication in human cells, but the reaction system is relatively simple.
　　 Of course, this does not mean that PCR is simple.
　　 On the contrary, PCR is very difficult.
　　 First, through high temperature "denaturation", a piece of double-stranded DNA is melted into two single-stranded DNA.
After "annealing", the "primer" and the template DNA are complementary combined at low temperature to form a partial double strand.
　　 In the end, it is "extended" in the middle of the fire. The DNA polymerase catalyzes the extension reaction of the DNA strand with the primer as the starting point to become two double-stranded DNA.
　　 Repeat this process continuously.
　　 1 becomes 2, 2 becomes 4, 4 becomes 8, 8 becomes 16, 16 becomes 32...
　　Through PCR technology, a sufficient amount of DNA can be amplified from a hair, a drop of blood, or even a cell for analysis, research, detection and identification.
　　 such as a paternity test;
　　Such as vaccine research;
　　 such as the prevention and treatment of viral diseases;
　　 or genetic disease research, etc., a lot of PCR is required.
　　 Of course, in the rice aphid biochemical reagent experiment that Zhou Wen did today, PCR is actually unnecessary.
　　Aphids are very low-level insects. Generally, drugs are used directly on them, and then you can just look at their genetic characteristics.
　　The reason for doing PCR is to learn PCR.
　　 PCR reaction system contains water, buffer, DNTP (deoxyribonucleoside triphosphate), primer P1/P2/DNA template and TAQ enzyme.
　　 After everything was ready, Zhou Wen adjusted the volume of the extraction gun, and then extracted 30μl of water into the three sets of PCR tubes.
　　 Strictly follow the operating procedures when aspirating liquid, vertical suction, slow suction and slow release.
　　 After changing the tip, I extracted 5 ml of buffer into the PCR tube.
　　 Then add DNTP/P1/P2/DNA template to the tube separately...
　　 Thanks to this period of study, except for a little nervousness at the beginning, Zhou Wen did it in an orderly manner.
After 　　 wait for the required raw materials to be added, the next step is to centrifuge.
　　 After setting the speed as required, after waiting for three minutes, the centrifugation was completed.
　　 Zhou Wen took out the PCR tube containing the mixed solution from the centrifuge and immediately put it on the PCR machine for amplification.
　　 set 94℃ pre-denaturation for 4min.
　　 Then set 20 cycles of amplification stage.
　　 high temperature, low temperature, medium temperature, high temperature...
　　 1 becomes 2, 2 becomes 4, 4 becomes 8, 8 becomes 16, 16 becomes 32...
　　 Zhou Wen waited anxiously, feeling like a husband outside the delivery room, praying silently for "Mother and child peace", and at the same time, wondering if the one who will be born is a boy or a girl? Are you healthy?
　　 At this moment, Zhou Wen suddenly thought that system integration could speed up the progress of the experiment. Instead of waiting here for a few hours, how about speeding it up?
　　 No one sees it here anyway.
　　 Thinking of this, Zhou Wen immediately summoned the personal attribute page. After clicking on the points, an option popped up.
　　【Will I consume points to speed up the progress of the experiment? whether】
【Yes】.
　　 [This task will cost 20 points in total, please confirm again]
　　 Zhou Wen sees that only 20 points can speed up the progress of the experiment, so there is any hesitation, so he immediately chose to confirm.
　　 Before Zhou Wen could react, the PCR machine had completed the amplification in the next second.
　　 Zhou Wen was surprised, the power of the system was simply mysterious.
　　 sighed in his heart, and soon he became nervous again.
　　I did PCR for the first time, I wonder if I can succeed?
　　 Zhou Wen turned on the PCR machine and took out the PCR tube from it.
　　 Originally, the following was product analysis, but Zhou Wen had real eyes and squinted his eyes slightly, and soon he saw the high-density double helix structure of DNA.
　　 "perfect~"
　　 If it weren't for wearing a mask, Zhou Wen would laugh out loud.
　　 There is one student who counts as one student. Who hasn’t learned to do PCR after countless failures? Can anyone like him, do amplification for the first time and succeed all at once?
　　 And the amplification effect is unexpectedly good, it is perfect.
　　……
　　 Jiangzhou City, First People's Hospital, UU Reading www.uukanshu.com Testing Center, PCR Group.
　　 Wu Yan had lunch outside before returning to the hospital, and then went to the team leader's office to report the situation as soon as possible.
　　 "What about people?" Zhang Shuguang, who was writing the experiment report, asked without raising his head.
　　 Wu Yan said with some embarrassment: "That... hasn't come yet."
When 　　 went, she patted her chest and promised that she was caught by her hand, and the cowhide was blown up.
　　 Zhang Shuguang raised his head and said unexpectedly: "Why, don't you want to come?"
　　Wu Yan shook her head and said, "No, but he said..."
　　 Wu Yan told Zhou Wen’s request to Zhang Shuguang.
　　 Zhang Shuguang took the pen with his left hand, tapped on the desk, and said with a smile: "You said you, such a smart person, why do you lose your chain at a critical time?"
　　 Wu Yan said strangely: "Why did I drop the chain? He wants a formal salary. How can I dare to go beyond this and promise you?"
　　Zhang Shuguang smiled and said: "What's the matter with Yue Chu, he is a biology undergraduate, and he dares to open his mouth and ask you to set up a career. Don't you want to think about why?"
　　 "Why?" Wu Yan was puzzled, and soon reacted, surprised: "Oh~~~You mean..."
　　 Zhang Shuguang laughed and said, "You've all come to find someone, as long as it's not too outrageous, you should agree to him first. If you have any questions, I will talk about it later. Verbal promises are not a package of votes, right?"
　　 "Uhhhh~ Boss, I know it was wrong." Wu Yan nodded and admitted her mistake, "Then I call him now?"
　　 Zhang Shuguang shook his head funny, "I'm back all the time, what's the hurry, I'm afraid he won't be able to run. Anyway, we are also Jiangzhou First Hospital, so we can't be underestimated, so I stretch him for two days before talking."