Chapter 190: Please don't bring me

2333, Jiangzhou Science and Technology Park, Cryptococcosis Research Institute.
It has been more than half a year since its establishment last year.
But as of now, the research has achieved nothing.
The reason has already been said before, it is too difficult to clone cells, because the content of antigenic b cells in serum is too low.
After B cells are isolated, the sensitivity and specificity requirements for sequence amplification and determination are also very high.
Even if the sequence is amplified and the antibody is expressed, the "neutralizing effect" of the antibody needs further identification.
In short, it is extremely difficult to replicate antigenic B cells.
Of course, the above are all for the average person.
Does not include Zhou Wen.
He has now upgraded to LV4 in several disciplines, and he can almost start trying to do this task.
This task brought Zhou Wen not only experience, but also other additional things, such as influence in academia, which other tasks could not bring him.
On the afternoon of May 5th, Zhou Wen officially started cell cloning research.
The first step is to isolate B cells.
This is extremely difficult for others, but for Zhou Wen, who has true vision, it is a piece of cake.
First of all, his eyes can directly see the antigenic B cells in the serum;
Secondly, the upgrade of biological immunology to LV4 brought him more than just a simple amount of knowledge, but also a deeper understanding of antigenic B cells, and he mastered a set of unique separation techniques.
These techniques are independent of existing biological knowledge.
A week later, Zhou Wen successfully isolated cryptococcosis antigenic B cells.
The shocked eyes of Li Feng and Fan Bo, who had always been very critical of Zhou Wen, almost didn't come out.
The other 13 doctoral researchers in the laboratory were also shocked.
Everyone's admiration for Zhou Wen is like a surging river, uninterrupted, and like the Yellow River overflowing, out of control.
But everyone did not cheer!
This is just the first step in the Long March.
Next is the highlight-"sequence amplification"!
This is also extremely difficult for ordinary people, because antigenic B cells are too rare, and the failure rate is as high as 99% during sequence amplification!
And if it fails, there will probably never be another chance.
But this is not too big a problem for Zhou Wen.
Don't forget, what did Zhou Wen start from? DNA extraction and amplification!
He thinks he is second in DNA extraction and PCR in the world, and no one dares to call himself the first.
Of course, Zhou Wen didn't dare to take it too lightly, he still cheered up and went all out.
Because there are too few specimens.
If it fails, the separated B cells will not be considered invalid, and it will take a week to separate again, and it may not be a one-time success!
It was similar to the mouse DNA amplification program, except that he redesigned the primers himself.
Many simulation experiments have also been carried out.
Six days later, Zhou Wen successfully amplified a sufficient amount of antigenic B cells, and after product analysis, it was determined that the amplification was successful!
There is no shortage of research samples now.
The entire human laboratory is boiling!
Everyone looked at Zhou Wen like a god.
If it is said that the isolation of antigenic B cells at one time belongs to the category of human beings, then it is completely non-human technology to amplify antigen B cells at one time without any operating experience and no chance of recurring failure.
At this moment, Zhou Wen has become their spiritual belief!
Even if he now says he is an alien, they are convinced!
Next is the antibody expression assay.
Antibody expression is a multi-step process that starts from the transcription from DNA to mRNA and reaches a climax when mRNA is translated into protein...
Save 30,000 words of introduction here.
Not surprisingly, the sensitivity of antibody expression is naturally a one-time pass.
Li Feng and other doctors were not surprised at all, and even thought it was a matter of course.
The next step is the most important step, and the final step is the antibody "neutralization effect" test.
Because the "antibody" itself only has the function of recognizing the target antigen, and does not have the function of killing or rejecting the target antigen.
And the "neutralizing effect" is that the antibody binds to the antigen to produce a precipitate or cell group, which is then swallowed by phagocytes, thereby killing the antigen!
If the neutralization test is successful, it means that the cryptococcosis vaccine has been successfully manufactured.
...
Xiangya Hospital, Office of the Director of Infectious Diseases.
Cheng Yanqing was writing an experiment report, and the phone on the desk rang.
She picked it up and glanced at it. It was Pan Renfeng, a member of the Virus Communication House and Director of the Infectious Disease Department of Harmony Hospital.
"What's up Director Pan?"
"Zhou Wen said that the antigenic B cells they expanded have been tested for antibody expression and will be tested for neutralization soon."
Cheng Yan was stunned for a moment, and then went into shock and said, "What did you say?"
"I said that Zhou Wen and the others have isolated and expanded the antigenic B cells of cryptococcosis, and then they will conduct a neutralization test."
"Impossible!" Cheng Yanqing said categorically, "I called the research institute at the beginning of the month. Basic research is still being done over there. It's impossible."
Pan Renfeng said: "This is not what I said, this is what Zhou Wen said himself, you can take a look in the group."
When Cheng Yanqing heard the words, he immediately opened the WeChat group, and finally saw Zhou Wen's speech.
It was the old house of the Xia family who asked Zhou Wen what he was doing recently, and Zhou Wen casually replied that he was cloning the antigenic B cells of cryptococcosis.
Then Xia’s old house asked him how he studied?
Zhou Wen said that the neutralization effect test will begin soon.
Zhou Wen's words blew up all the big guys in the group who were diving and watching the screen.
Everyone can't believe it.
But after Zhou Wen published a few test data sheets, Qunli suddenly fryed the pot.
Cheng Yanqing clicked on the test data and looked at it, and he was shocked to no avail!
She wondered if today is April Fool's Day?
How can such a thing exist?
She called Li Feng at the beginning of the next month, and he sighed that there was no progress. His words revealed complaints against Zhou Wen.
But now...
Cheng Yanqing immediately called Zhou Wen, who was on the phone.
Cheng Yanqing did not hang up, but waited.
After about tens of seconds, the call was connected.
"Zhou Wen, what is going on?"
"Um... I won't be able to explain it clearly in a while, so let's, Director Cheng, if you have time, come to Jiangzhou tomorrow and talk in person."
Cheng Yanqing had too many questions in her heart, but at this moment she didn't know where to ask, so she had to hang up the phone for now.
How could Yanqing wait until tomorrow before going there?
Without a word, he immediately left for Jiangzhou.
Called the head of the laboratory Li Feng over and over on the road.
Finally got through.
Under Li Feng's narration, Cheng Yanqing's chin almost fell off, and he could not recover for a long time.
"Dududu"
Finally, Cheng Yanqing was awakened by the sound of a horn behind him, only to realize that he had stopped at the intersection of traffic lights.
After starting, Cheng Yanqing took a deep breath and asked, "Why don't you tell me anything about such a big thing?"
Li Feng said: "Because Zhou said that the process is not important, but the result is important. If the neutralization effect fails the test, all the previous sweat and efforts are meaningless, so there is no need to let everyone know."
Cheng Yanqing: "..."
Is this talking human?
The successful isolation of antigenic B cells is a remarkable feat, and it is also a very important reference for the isolation of antigenic B cells in other rare diseases!
In her opinion, this technology alone is worth three lancets!
How can it be said that it is meaningless?
As for the subsequent sequence amplification, it is also very important.
"This little guy is so shameless!"
Cheng Yanqing said something and couldn't help but laugh.
...
May 26, 2019, Sunday.
At 9:30 in the morning, the Jiangzhou Cryptococcal Disease Research Institute conducted a publicly disclosed cryptococcosis antigenic B cell neutralization test.
Some good friends and leaders familiar with Zhou Wen visited the site.
Such as Pan Renfeng, Cheng Yanqing, Gu Yunwen, Mu Yuanqing, Lin Huaidong, Zhang Wen, as well as several leaders of Jiangzhou Medical College and respected experts and professors.
The result was no surprise.
Under the attention of everyone, the cell group produced by the combination of the cryptococcosis antibody and the cryptococcosis antigen was swallowed up by the phagocytes!
"Papa Papa--"
In the observation room outside the laboratory, there was warm applause!
"Congratulations, Director Cheng, you are really amazing."
"This is an amazing invention! Director Cheng, you are so good!"
"..."
Facing the compliments of a group of old friends, Cheng Yanqing laughed and said: "If you do it, you don't want to praise here! I am also a leader. All research work is done by Zhou Wen, which has nothing to do with me. ."
The experts at the scene knew what was going on, so everyone smiled.
It doesn't matter if friends make jokes, but they are not so cheeky if they really want to tear their faces and grab the academic achievements of others!
And this one can't be taken away at all.
Several of the core technical processes in it, when the experimental recovery cannot be done, the shame will be lost to the grandma's house.
Experts are embarrassed, but one of the leaders of Jiangzhou Medical College laughed and said: "Director Cheng is too humble!
Xiao Zhou's outstanding ability is undeniable, but we can't ignore the role of leadership. Without the leadership's guidelines and proper scheduling, their young people can only spin around like a headless fly, right? "
Seeing this leader's serious look, Cheng Yanqing was embarrassed by UU reading www.uukanshu.com, and said to himself: "I'm not humble, so please brag and don't bring me!"
Seeing the expression on Cheng Yanqing's face, Pan Renfeng, Lin Huaidong, Zhang Wen and others couldn't help laughing.
But the big-bellied Jiangzhou Medical College leader was inexplicable in his heart, and said to himself: "Are my words funny?"
But he didn't dare to question, let alone show his face.
Pan Renfeng, Gu Yunwen, Lin Huaidong, Zhang Wen and others, which one is not a bigwig in the domestic medical system?
It is possible to talk directly with the head above.
At his level, it is not enough to see...
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