Chapter 4: mission completed
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Supreme Pharmacist System
- Second General
- 1480 characters
- 2021-03-03 11:12:45
Zhou Wen Follow the experimental procedure and pipette the supernatant.
"Blowing" is an academic term, which is to extract the supernatant with a test tube gun and then blow it out. The purpose is to fully stir the clear liquid in the test tube evenly.
pipette back and forth several times, and then extract the two DNA samples into the centrifuge to start centrifugation.
The centrifugation is complete after fifteen minutes.
Take the supernatant of group A to the side of the electrophoresis machine and add it from the gel well.
Cover the electrophoresis tank cover, select the electrophoresis voltage and direction, turn on the power switch, and start electrophoresis.
Followed by group B, the supernatant was also extracted, added from the gel well of another electrophoresis machine, and electrophoresis was also run.
Then there is a long wait.
The computer will compare the extracted DNA with the mouse genes in the "NCBI Gene Bank" in the United States one by one, and it will take at least 6 hours.
Because there are 2.5 billion DNA base pairs on the 20 pairs of chromosomes in a white mouse, it takes a long time.
During this time, you can read novels, play dramas, and eat melon seeds.
But Zhou Wen has no time.
went to catch a little white mouse and observed it with the eyes of true vision.
What he didn't expect was that what appeared in front of him this time was an introduction, [rat name: white mouse, mole rat variant. Gender: female. Rat age: 11 months. 】
"Hey, why did you see this?" Zhou Wen turned his head and looked at Lin Jiayi, who was facing away from him. The scene he had seen before appeared again.
【Name: Lin Jiayi. Gender: Female. Age: 24. Occupation: Biology student. body condition:? ? ? Mental state:? ? ? 】
He thought of the way to observe DNA, his eyes narrowed slightly, and soon the picture in front of him began to become clearer and clearer...
"Fuck...fuck, fuck..."
Lin Jiayi's white lab coat on the upper body slowly turned into fibrous tissue in his eyes, spreading, increasing... and continuing to move forward, it was a white chemical fiber fabric.
The pores of the chemical fiber fabric became thicker and thicker in front of the eyes, and finally passed through this layer of chemical fiber fabric and turned into a black cotton fabric.
Next is the epidermal keratinization layer, dermis, arteries, veins, hair follicles...
Just then, Lin Jiayi suddenly turned around and asked, "Zhou Wen, what are you looking at?"
Zhou Wen was interrupted, the picture in front of him disappeared, and he rubbed his nose and said: "Uh... you didn't look at me, how do you know that I was looking at you?"
Lin Jiayi pointed to the stainless steel frame of the sterile operating table next to it, and Zhou Wen's reflection can be clearly seen on it.
Lin Jiayi smiled and asked: "Hey, Zhou Wen, you are so kind to help Shi Lei in the experiment, isn't it just to stay with me?"
Zhou Wen: "...Why do you have such unrealistic ideas?"
Since Qu Fangfang left, Lin Jiayi probably felt that no one in the laboratory could threaten her appearance, so her words became more and more vain.
Of course, Lin Jiayi looks good. She wants a cheeky face, a body shape, and a small white lab coat is tightly stretched.
is a group of reality, one forehead thinks to find a rich and handsome boyfriend.
And it's too calculating, a bit disgusting.
Lin Jiayi smiled and said, "Hey~ it doesn't matter if you don't admit it... Forget it, I have a boyfriend, and you are too late even if you want to chase me."
Zhou Wen turned around and snapped off the mouse’s neck. He broke his stomach and said with a smile: "Congratulations on taking off the order. Tell me, what's the situation with the other party?"
"He is also from our school, one year younger than me, a native of Jiangzhou, and he studied CS..."
Just as Lin Jiayi was talking, Zhou Wen focused most of his attention on the mouse in his hand.
He found that when he took out the bone marrow from the white rat's thigh, he could already see the DNA chain.
This is much easier to handle, he can see the possible problems in the DNA extraction process more intuitively.
Soon he discovered that in the process of flushing with low osmotic fluid, a large amount of DNA fragments was lost.
I don’t know if it is caused by the low osmotic fluid concentration?
But fortunately, it does not affect the extraction of genes, and the concentration issues will be studied later.
Next is the counterweight centrifugation.
Zhou Wen, a plant-by-plant centrifuge test.
Soon he found the reason for the failure of extraction of group B, which was the problem of DNA extraction.
This is a common problem for novices. Others such as temperature control, time control, techniques, etc., will affect the number of DNA strands extracted and the success rate.
Under normal circumstances, there is no way. There is a difference between left-hand extraction and right-hand extraction. Centrifugation is one second or less, it may fail, and higher and lower temperatures will also have a great impact.
Because DNA is invisible to the naked eye, novices can only find out the possible problems through repeated experiments. That is to say, rely on "experience".
But now that Zhou Wen has real vision, he can see the DNA directly, but the result is completely different.
He stepped through the process of accumulating experience and became a top expert directly...
……
Time passed quickly, and it was already five thirty in the evening.
Lin Jiayi stood up and stretched her waist, beat her neck and walked to Zhou Wen's side and said, "Hey Zhou Wen, do you want to eat together?"
Zhou Wen, who was "distributing glue", said without looking up: "You go first, I will be ready soon."
Lin Jiayi didn't notice, there was a layer of mouse corpses piled up in the waste bucket next to him, and nodded and said: "Okay, then I'm leaving."
"Ok……"
The electrophoresis analysis was not completed until 7:30 in the evening.
After comparison, it was found that the extraction of group A was successful, while group B was not unexpected. The data was seriously unreasonable, indicating that the extraction really failed.
Zhou Wen looked at the data chart, and smiled uncontrollably at the corner of his mouth.
task required 6 gene fragments, and in the afternoon he extracted a total of 8 strains, of which 3 strains were obtained after electrophoresis analysis, 1 strain was obtained after electron microscope observation, and 4 strains were identified by naked eyes.
At this moment, a soft voice rang in my ear, just like a voice actor.
[The conditions of the task have been fulfilled, may I submit the task immediately? 】
After Zhou Wen was shocked, he was immediately overjoyed and immediately opened the pharmacist system.
On the blue property board, a prompt box pops up, and it says [Do you want to submit the task immediately? whether】
Weekly selection [Yes]
【Ding! mission completed. UU reading www. uukanshu.com is now giving out rewards... Congratulations on gaining 100 subject experience and 200 points. 】
After giving out the experience, a suggestion box popped up again, [Excuse me, open the box now? whether】
Zhou Wenlai was slightly excited, and became excited as the opening prompt popped up.
"God bless you, open a million...no, 10,000 will do. It's not enough for 1,000 yuan."
He is really short of money now, and all his belongings are left with 100 yuan.
Today is only March 19th, and the 100 yuan must be held until the beginning of next month.
In fact, when he came to school in the next year, he had two thousand yuan on his body.
As a result, the phone accidentally broke. I spent 1,000 to buy a three-handed 7P from my younger brother, and then recharged another 600 meal cards. I bought some things piecemeal, and only 100 yuan was left.
Zhou Wen prayed silently in his heart, and then chose [Yes]
Just after choosing, a black lacquered wooden texture treasure chest popped out, with complicated patterns carved on it, and an ancient key floating beside it.
"It's kind of like that~" Zhou Wen rubbed his hands in excitement, and then muttered: "The emperor is possessed by the emperor, and the non-chiefs avoid..."
【On】
With the words, the key floating next to the treasure chest is automatically inserted into the keyhole of the treasure chest.
After rotated a circle, the treasure chest opened slowly, and an object shining with white light emerged.
Before Zhou Wen could see clearly what it was, a prompt box popped up.
He took a closer look, [Congratulations, you got the bio-type N99 mask×10...]