Chapter 59: penicillin

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Back in the courtyard, Yan Yan will see Xing Niang sitting under the gallery cutting the fabric, looking awkward, sighing for a while.
When Xing Niang saw Yan Yan coming back, she quickly came up, took over the power fence, looked at the face of Yan Yan, and said with a distressed heart: "Is my mother tired? Go back to the main house in a few days, find someone who is ill. There will be a lot less."
"Well, Xing Niang, help me to bathe soup, I want to bathe." Yan Yandao.
Xing Niang smiled and said: "I have already prepared it, it is the bathing to go to the sick."
It took a long time for Yan Yan to recover. It was originally empty. On this day, two patients were diagnosed and two bodies were dissected.
Soaking in warm water, the face is a little drowsy.
"Mother, Xing Niang ordered slaves to wait for you to bathe." A clear voice sounded outside.
Yan Yan is not used to being watching her taking a bath next to her, but this time she is really sleepy. She can help the aunt to wash the long hair and say: "Come in."
The person came in the morning to make a small dress for Yan Yan. She had a small bath tub with a wooden cover on it. The gap was faintly hot, and a floral fragrance dissipated in the air.
"What is it?" asked Yan Yan.
Xiaoman knows that Yan Yan is not as scary as the surface looks like, smiles shallowly and opens the small dimples on the cheeks. "The slaves see the honeysuckle in the courtyard, so they took some baths for the mother and honeysuckle in the summer. The bath is very good!"
Yan Yan nodded, but thought in her heart, it was really right for Xiao Yu, the spring is probably being killed in the bath tub, but... Who is the bath in which she died?
Xiao Man saw that Yan Yan did not speak, then dared to go forward: "Mother, slaves help you wash your hair?"
"Good." Yan Yan should be.
Xiaoman pulled a high stool from the corner of the wall and placed it on the side of the tub, then put the small bucket on it.
Yan Yan drove to the body while watching the action of Xiaoman, the pupil slammed into a high stool.
The high stool in the bath room was a bit rough, but it was very similar to the stool that fell to the ground under the green day. The Tang Dynasty people were still sitting, the cases were very low, and there were similar legs. The seat of the chair is only a bed of sand, and the bed is far less.
Also, this bucket cover...
"Xiaoman, you stand opposite me." Yan Yan slowly said.
Xiaoman is pouring the flower bath soup into the tub. Hearing that Yan Yan remembers her name, she will happily respond, and will happily put the small half bucket of water on the high stool on the side of the tub. To the opposite.
Yan Yan stretched out his hand from the tub, touched the small wooden bucket, and poured all the water inside into the tub. Then I began to study the barrel of the wooden barrel, as well as the lid of the bucket. From time to time, holding these two things on the head of the small head, I saw a small tremor in my heart, for fear that she really threw things on her head. .
The barrel mouth is narrow at the bottom, but it is very heavy. No matter whether it is the bottom or the mouth, it will not just edema. At least it has to be broken. The wound on the head of the spring is curved. It should be caused by the lid. .
The size of the barrel is different, and the arc is different. About the murderer is holding the bucket lid on the spring surface, and then immediately discovers that she is not dead, nor fainted...
Yan Yan slammed out of the bucket and reached for the neck.
Xiaoman stunned and looked at the body of Yan Yan bumps, the skin of the sheep fat jade hangs in the smog, standing in the smog, simply screaming.
She looked at the gods, but her neck was slammed forward, and she fell the edge of the tub. Her face was almost attached to the water. She hurriedly shouted: "Niang Niu Niang is forgiving!"
Yan Yan returned to God and found herself naked standing in the tub, coughing and cried, sitting silently back in the water, explaining to the shocked Xiaoman: "I... just think of something."
Hearing such an explanation, Xiaoman couldn’t let go of his mind, thinking about something, and then holding the bucket cover to her head, and then wanting to press her into the water?
But... the mother’s body is really worth seeing! The eyes of Xiaoman Shui Ling Ling glanced at the looming body in the water, whispered: "Mother, slaves wash your hair."
Yan Yan nodded, and then figured out some things, no longer toss, let Xiao Man help her to brush a long hair and waist.
After I finished bathing, Yan Yan immediately went back to the room to grind, painted the shape of the tub and the appearance of the high stool, and then wrote a letter to the team guarding Yang Zhuang, let him immediately Send someone to Liu Zhishi.
After doing all this, Yan Yan was really relaxed. After using the dinner, she went to the green for a while and then went back to the dormitory to rest.
Xu is so tired that there are many pictures in this night's dream, but after all, it has never been a period of time. It is the best time to sleep in these six or seven years.
In the next few days, it was very rainy and rainy, and it was very inconvenient to travel. Anyone who asked for a diagnosis of the disease had sent a letter saying that she would not bother to go to the door and bring the patient to the door on the agreed date.
So these days, Yan Yan was idle. After the patient was diagnosed every day, he went to Wu Xiu and listened to him for medical treatment. Since Yan Yan had made the meal, Wu Xiuhe was obviously more focused and harder than before. From time to time, it was clear that she should make a meal and work hard.
The time was unconscious for five days, and the penicillin placed in the medium was almost ready to extract penicillin.
Yan Yan pours the liquid from the ten petri dishes into a specially prepared wooden barrel, then injects the vegetable oil into the barrel and mixes and stirs.
Penicillium water contains a fat-soluble substance, and the oil can dissolve and separate the fat-soluble substance. After stirring evenly, it is left to wait.
Eventually, the contents of the barrel will be separated into layers. The first layer is a fat-soluble substance dissolved in oil, and the second layer is an insoluble substance that is insoluble in water and insoluble in oil. Because penicillin is a water-soluble substance, Penicillin is contained in the water that sinks to the bottom.
The special wooden barrel is made into a casks. There is a cork, the cork is pulled out, and the bottom water is slowly flowing out. The enamel is connected to a porcelain enamel sterilized with wine and distilled water.
After filtering some of the large impurities, pour into the altar, which is filled with sterilized broken carbon. After stirring, penicillin is absorbed by the carbon.
Xing Niang, who was helping her side, looked at her face and looked at the pile of bottles and cans. She didn’t understand that there was a mold in the bucket, and she placed the water for seven days. What would be different after the oil was too carbonized?
In fact, these steps of today's operation are not difficult at all. What is difficult is that the preparation of these materials, such as disinfecting wine and distilled water, makes Yan Yan busy and busy every day, especially the collection of distilled water, although It's not difficult, but the amount is small. It takes a lot of time to collect a bottle. When disinfecting, it will dump most of the bottle. It is just the blood and sweat of the face.
There is also sodium carbonate required for filtration, commonly known as alkali and soda. It should be made from salt, sulfuric acid, coal, and limestone. Fortunately, Yan Yan has been immersed in the father of the family for several years. Otherwise, he knows how to extract penicillin. Can only blink dry.
After mixing the carbon and water-soluble substances, the surface is purified and added separately: distilled water, baking soda water, weak acid water (vinegar + water).
The first pass is added with distilled water to filter, and the poured water is not a penicillin solution, and can be discarded.
In the second pass, weak acid water was added and the filtered water was still thrown away.
In the first step, adding baking soda water, the filtered solution is the real aqueous solution of penicillin.
Yan Yan looked at the four bottles filled with an aqueous solution of penicillin. She was always calm, and her eyes were slightly reddish – it was not easy! These days, just getting distilled water is so tired that she has back pain.
When I thought about seeing the syphilis in Zixu, Yan Yan didn't think much, because as a modern modern person, a modern medical practitioner, the subconscious mind felt that the penicillin extraction procedure was relatively simple, but it was really known. There is no soda in the Tang Dynasty. There is no distilled water. The fine carbon should be ground slowly. The medium solution should be cooked with steamed bread and rice.
How can there be such a difficult disease in Datang! Yan Yanxin sighed, if she could change it, she would rather dissect two bodies every day and would never be willing to make penicillin.
"Mother, this is the fairy water?" Xing Niang asked in a whisper.
When Yan Yan paused for a moment, he remembered that when he said that someone was pointing in the dream, he nodded.
Yan Yan poured two drops in another Petri dish, which contained Staphylococcus aureus. This strain is better. After a few days before the sputum, I found it in the culture solution to promote its growth. Today, there is It’s full. Staphylococcus aureus gradually disappeared with the instillation of an aqueous solution of penicillin, and the place where it was dropped quickly emptied.
Verification is useful, Yan Yan completely relieved a sigh of relief, if it costs a lot of effort, this last step is also verified to be invalid, then she really wants to cry, but fortunately she is not mean to treat her.
Yan Yan carefully put away these bottles of hard-won penicillin, a big stone in my heart finally put down, feeling quite relaxed, decided to make a meal to the cheap Master.
Wu Xiu and his stretched neck waited for a long while, and Yan Yan had just made the first dish. Yun Yunsheng came, and he was so angry that his old man was blowing his eyes, and the sacred bones that were hard to maintain were instantly collapsed and shattered. Do nothing.
In the face of helplessness, he had to make a piece of his favorite Dongpo meat, one by one, to comfort his broken heart.
Yun Yunsheng laughed like a flower, "A Yan, your master is really interesting."
Yan Yan’s lips were slightly bent. Instead of picking up this topic, he asked:
Are you busy?

"Have you forgotten?" Yun Yunsheng paused and groaned: "Today is the Qixi Festival! How can you forget about being a daughter!"
"Oh." Yan Yan remembered that Xing Niang had been busy since the early morning.
"Come on, go to the grooming, we will go to Pingjiang River tonight, and the uncle will let me take you there!" Yun Yunsheng hurriedly urged.
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