Chapter 3: True Sight
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Supreme Pharmacist System
- Second General
- 1715 characters
- 2021-03-03 11:12:45
dander under the electron microscope, no abnormalities were found.
Two-thirds of the big stone in Zhou Wen's heart was dropped.
But he has to wait for the mutant aphid to be tested tomorrow, and he can be completely relieved after he is sure that there is no virus.
After I was busy with things here, it happened to be lunch time.
Zhou Wen came to Shi Lei and asked with a smile: "Brother Lei, do you need help?"
Shi Lei, who had just made the electrophoresis gel and was about to kill the mice, said without raising his head, "What's the matter, are you finished?"
Zhou Wen said with a smile: "I'm so busy, just change the data later, you know."
It usually takes 2 to 3 days to run an electrophoresis process. In addition, PCR has to be done for two or three days, and the experimental data is often found to be seriously unreasonable.
Then there are fck, Jesus, damnit, a scream.
If you do not plan to do it again, you can only fake the data.
This is basically an unspoken rule of biological life, most biological life has done this.
Shi Lei chuckled twice. He was also the person who came over, nodded and said: "In this case, you should go back and rest first. I can do it myself. By the way, bring us a meal."
Zhou Wen turned around, leaning on the test bench and said, "Why are you going back, there is no girlfriend."
Lin Jiayi, who was wearing a white coat and looking at the microscope with her back to them, turned around and said gossiping: "Ai Zhouwen, are you still in contact with Qu Fangfang?"
Qu Fangfang turned out to be the most beautiful among the few girls in the laboratory, with a gentle personality, Xiaojiabiyu, and an ideal wife in the eyes of boys.
It's a pity to go for an internship after the year.
Zhou Wen replied: "I haven't contacted, what's the matter?"
"Nothing." Lin Jiayi said casually, and then giggled and said: "Hey Zhou Wen, you invite me to dinner, how about being your girlfriend?"
Zhou Wen curled his lips and said: "As long as you women have someone who can speak up, my child will be soy sauce now."
Shi Lei laughed straight.
Lin Jiayi also turned around smilingly.
It's just a joke. She is already a second-year graduate, and she knows what kind of boyfriend she should look for.
Here, Zhou Wen turned his head and smiled: "Brother Lei, should you help? If you don't want me, I will go back to play the show."
When Shi Lei heard that he was going back to watch the show, he looked up and said with a smirk: "Then you should stay. It happened that Lan Lan asked me to go to the movie in the afternoon."
Zhou Wen urged: "Then you don't have to go back and pick up, try to get a successful intubation at night."
Shi Lei smiled naively.
Similar to Zhou Wen, Shi Lei's family conditions are not very good, plus he looks like a blind monk, and he can't speak sweetly. He is almost graduated from graduate school, and has never kissed a girl.
It's rare to meet a girl who doesn't dislike him, everyone is very happy for him.
also recognized after receiving the plate.
"Then I am really leaving?"
"Go ahead."
Shi Lei handed the mouse in his hand to Zhou Wen, turned around and left the laboratory.
"Remember to bring us food."
"I know..."
Here, Zhou Wen pinched the mouse’s neck with one hand and pressed it on the operating table, while the other hand grabbed the mouse’s tail and lifted it up, with a "click", giving the mouse a "cervical decapitation." law".
There is no pain at all.
Then quickly opened his stomach, cut the mouse's thigh bone with scissors, and peeled off the muscle and fascia outside the leg bone.
After rinsing, cut the thigh bones and extract the bone marrow, followed by flushing the thigh bone marrow with a low osmotic fluid, and finally put the rinsed "washing fluid" into the test tube in equal parts for each plant.
Then another white mouse was strangled to death. After the bone marrow was drawn, the washing solution was prepared step by step according to the above steps, and the previous washing solution was matched with the weight according to the AB group, and then the centrifuge was started to centrifuge.
The genes of mice are 95% similar to human genes. Therefore, many large laboratories use mice genes for various drug tests.
However, after a gene extraction process, it usually takes 2 to 3 days. Those large laboratories and research institutes that pay attention to efficiency will hand over the gene extraction work to the laboratories of various universities.
A mouse can theoretically extract 4 to 8 gene fragments, but normally 4 strains are extracted.
Of course, these are charged.
The current market price of a plant is about 400~500.
……
Just after centrifugation was completed here, Shi Lei came back with a packed meal.
After dinner in the office outside, Zhou Wen was going to do electrophoresis analysis, commonly known as "run electrophoresis".
I have been exposed to this in high school.
is to use agarose gel to make a gel plate, so that the sample is electrophoresed on the gel plate, so it is also called "run gel", which can be used to detect DNA strands.
In addition to running electrophoresis, another method is electron microscope observation. This saves much time compared to running electrophoresis.
However, for electron microscopy observation, it is necessary to prepare a glass slide and stain the gene. In this process, time, technique, temperature, and possible contamination may lead to extraction failure, and waste of previous efforts.
So novices generally run electrophoresis honestly.
Zhou Wen took the extraction gun and prepared to extract the supernatant and then perform "pipetting". At this moment, he suddenly thought of "True Sight".
Want to see how True Sight introduces DNA?
He raised the test tube in his right hand and shook it twice against the light in front of the operating table. At the same time, his eyes were slightly narrowed and he stared at the liquid in the centrifuge tube.
What made Zhou Wen couldn't believe was that the image of the white mouse's DNA chain appeared directly in front of his eyes, including the broken covalent bonds.
"My Nima..."
Zhou Wen almost didn't jump up with excitement.
His eyes can see DNA clearly. Doesn't that mean that his eyes have caught up with the electron microscope?
He immediately put down the centrifuge tube in his hand, took off the rubber gloves and opened his hands, his eyes narrowed slightly, and he imagined the "Eye of True Vision" in his mind.
"Hush", his hand is like a glass slide under the microscope, clearly.
But in the next second he won’t be happy anymore, and he saw Staphylococcus, Staphylococcus aureus and long strips of E. coli all over his palm...
There are other kinds of bacteria and microorganisms crawling around in the palm of the hand.
In addition, the inside of the nail is like an old nest of bacteria. It is packed with all kinds of bacteria. It looks very disgusting.
I have washed my hands. I really don’t know how many bacteria there will be without washing my hands?
"Fucking~" Zhou Wen flicked his hands twice in fright, and then remembered that the bacteria can't be shaken off, so he hurried to wash his hands again.
He knows that there are a lot of bacteria on human hands, but they are all made with bacteria-made films and then observed under a microscope.
Because the hands are opaque, there is no way to see clearly.
Because of this, he has never had such an intuitive visual shock, and for the first time he realized the importance of hand washing.
Zhou Wen rolled up his sleeves, first washed his hands with an alcohol-based hand sanitizer, then found a nail clipper, carefully trimmed the dirty nails, and then carefully washed his hands again.
Then use the eyes of true vision to read, UU reading www.uukahnshu. More than 90% of the bacteria on com's hands have been eliminated, and only a few stubborn bacteria are still attached to the fingers and skin folds.
Zhou Wen said silently: "No wonder experiments often fail. It's only because of the number of bacteria on hand that the success rate is high."
Back to the laboratory, I looked at the rubber gloves on the table with my true vision, and found that they were also covered with bacteria.
"Huh~" Zhou Wen picked up the inoculation rod on the side table, picked up the gloves and threw them into the waste bucket, and then found a pair of sterile gloves to put on.
After everything was reset, Zhou Wen excitedly picked up the group A centrifuge tube containing the mouse gene fragments and observed again.
No accident, a clear DNA image appeared in front of him.
This shows that the mouse's gene extraction was successful.
I ran electrophoresis and electron microscope observation, the purpose is to detect whether there is DNA in the test tube.
And now that he can see the DNA directly, it can be said that the most cumbersome and the easiest to fail procedure is eliminated.
Then he picked up the centrifuge tube of Group B and looked at it, but this time he did not see the DNA strand.
Zhou Wen secretly said: "Did the extraction fail?"
After putting it down, he picked up another tube of clear liquid from group B and squinted his eyes, but still did not see the DNA strand.
He picked up one of the tubes of Group A again, but this time he saw it clearly.
This also shows that Group B really failed the extraction.
"Obviously all the processes are exactly the same, I don't know which link went wrong?" Zhou Wen frowned in thought.
He decided to do an electrophoresis analysis first to see if what the Eye of True Sight saw was real, and then to study the errors in the operation link...
Ps: I know I was wrong, please forgive me.