Chapter 355: Is this a major breakthrough?

After removing the specimen from the human body, Lu Li walked out of the operating room.
Coming to a test stand next to him, Lu Li sat down and began to do T cell gene editing.
Gene editing and modification, this technology has appeared long ago.
Gene modification by natural means, fruit tree grafting technology, and even hybrid rice technology that people are familiar with are all genetic modification technologies.
People are also familiar with gene editing and modification by unnatural means, such as...transgenic technology.
Even if it is done on human genes, it is not once or twice.
As early as 2016, a guy named He Jiankui worked on human embryos to create a pair of so-called "immune AIDS" gene-edited babies, and then...He Jiankui was arrested and went to jail!
In the case of completely immature technology, how to make a pair of gene-edited babies... Is there any scientific conscience and morality?
If genetic technology is a great way to the sky, humans have just taken a small step, do they want to play God? How stupid and arrogant is this?
The more advanced human science and technology, the more we must maintain awe.
Sitting in front of the test bench, Lu Li carefully took out a sample of cancer cells and installed it under an electron microscope.
In the electron microscope image, the internal structure of the cell is clearly presented on the display screen.
The endoplasmic reticulum, ribosomes, Golgi apparatus, lysosomes, mitochondria, microsomes, vacuoles, cytoskeleton, cytosol, and even the DNA entangled inside the nucleus are clearly presented.
DNA needs an electron microscope to see, but... if you straighten it, the DNA chain is more than two meters long.
Manipulating the instrument and using a gene gun to inject Cas9 transcriptase into the cancer cells, Lu Li clearly saw that the transcriptase was close to the DNA chain and copied a piece of DNA information.
The transcription of cancer cell gene information is completed.
Lu Li started the next experiment again. Install the T cells under an electron microscope and use a gene gun to inject the genetic information of cancer cells into the T cells.
Soon, T cells absorbed this genetic information and incorporated it into the "hostile sequence" of the immune system.
With this step, a weapon against cancer cells has been prepared.
But... Once injected into the human body, the T cells split crazily, which will inevitably bring the immune system to go crazy and kill them regardless of the enemy.
The result is that the immune system kills the human body before the cancer kills the human body.
Therefore, Lu Li had to do one more step.
This time, Lu Li planned to modify the "information dissemination mechanism" of the T cell so that it would no longer call for "air support" and would no longer "call allies" everywhere, but only let it fight alone.
Perhaps, this way it may not cause the immune system to go crazy.
This involves another gene editing technology, CRISPR-Cas9.
This gene editing technology is derived from the mutation mechanism of bacteria against bacteriophages.
When bacteria resist the attack of phage, they will mutate into a special protein, which can cut off the gene chain injected by phage into the nucleus.
I have to mention here that bacteriophages are the most terrifying killer in this world.
At every moment, in every corner of the world, there is a war that has lasted for countless years. The scale of the war reached a horror on the order of billions of trillions.
This is the attack of bacteriophages on bacteria.
A bacteriophage is a virus. It has a very regular appearance. The head is a twentieth-sided dice, with a small stick-like torso, and a few spider legs below it, just like a nano-robot.
Bacteriophages are harmless to the human body, they just attack bacteria frantically. They inject their own DNA into the bacteria, split into countless phages inside the cells, thereby killing the bacteria, and continue to spread.
In the face of the attack of phage, bacteria have also evolved a means of resistance, which is a special protein mentioned above. This protein cuts off the DNA injected by the phage.
Humans use this special protein to cut genes, modify genes, and even edit genes.
In the laboratory, Lu Li repeated the experiment of bacteriophages attacking bacteria and obtained the special protein CRISPR used by bacteria to fight bacteriophages.
Then the CRISPR protein, combined with Cas9 transcriptase, was injected into T cells to modify the genes of T cells.
Gene mapping is a problem.
Lu Li did countless experiments in a row, but failed to obtain accurate gene modification effects.
Even if the transcriptase will make a genetic match, but... the cut protein often cuts the wrong place.
Manipulating genes is not about cutting with scissors. This kind of extremely microscopic operation, no tool can penetrate into the nucleus, and it can only be achieved by cutting the protein.
Fortunately, the gene matching of transcriptase is still in play.
After repeating the experiment hundreds of times, I finally got the right place once.
A T cell after gene editing was finally born.
Lu Li hurriedly got up and injected this T cell into the virtual human body.
Then just wait!
After gene-edited T cells entered the human body, they immediately detected the presence of "hostile target" cancer cells, instantly activated the stress mechanism, began to absorb human nutrients, and split wildly.
Half an hour later, the experimental human body began to have a fever, and the heart rate increased sharply.
This is the immune mechanism at work. The symptoms of fever are the normal mechanism by which the immune system responds to virus invasion.
Then... the immune system is still crazy!
Even if T cells do not "call allies", the deployment of a large army of T cells will still cause the body to produce a violent immune response, and the "factor storm" still occurs.
It's just... it seems that the scale is not that serious.
Lu Li hurriedly virtualized drugs to control the madness of the immune system to maintain the vital signs of the experimental body.
Heart-strengthening acupuncture, adrenal hormones, first aid, and all kinds of emergency methods have been used in succession. I don't know how long it took for this experimental human body to stabilize.
"Sure enough, my thinking is right!"
Lu Li let out a long sigh and grinned, "Eliminating the ability of T cells to call allies can reduce the chance of the immune system going crazy."
Although this little progress cannot make a key breakthrough in CAR-T cancer treatment technology, but... as a postgraduate thesis, the gold content is absolutely sufficient.
After closing the virtual laboratory, Lu Li opened his eyes, and another night passed.
Woke up to wash, went out to eat breakfast, Lu Li returned home, sat in front of the computer, and began to write thesis.
"CAR-T technology, a preliminary exploration of the mechanism of shielding T cell information transmission!"
Sitting at the computer desk, Lu Li cracked his hands on the keyboard. A paper with detailed data, clear experimental procedures, and a true and reliable paper was easily written.
One Chinese, one English, all done.
Then, Lu Li called Professor He, "Teacher, my research topic is already..."
"Already thought about the subject? It's very efficient!"
Before Lu Li could finish speaking, Professor He took over, "Okay, you can send me the subject! I'll report it to you."
"That... isn't a topic, it's a paper. I wrote a paper."
Lu Li quickly explained: "Teacher, I wrote a paper and sent it to your mailbox. Please correct it!"
"paper?"
Professor He was stunned for a moment. You didn't even choose a topic, so you wrote the paper? Is it the kind of irrigated essay with a mixed name?
"I will take a look!"
Professor He didn't say much, he was going to watch it before saying it. If it is really irrigating the paper, then you have to clean up Lu Li and kill this evil trend.
Sitting back at the office desk, Professor He turned on the computer and saw in the mailbox the paper sent by Lu Li.
Clicking on the paper and seeing the title of "CAR-T Technology, Shielding T Cell Information Transmission Mechanism", Professor He was taken aback.
CAR-T technology? This is still the world's cutting-edge scientific research project!
What is the information transmission mechanism of screen T cells? New gene editing technology?
Professor He suddenly looked serious, and looked at it seriously.
The paper was in duplicate, one in Chinese and one in English. Professor He didn't bother to read the English version, so he picked up the Chinese version and read it.
The whole paper has no moisture at all, it's all dry.
In the paper, Lu Li gave a detailed description of the new gene editing direction of T cells. Each step is clear and the experimental data is also very detailed.
From the perspective of the paper, as long as Lu Li's experimental procedures are followed, this gene editing technology that shields the information transmission mechanism of T cells can be completely repeated.
From a theoretical analysis, shielding the information transmission mechanism of T cells can indeed effectively reduce the "factor storm" and reduce the chance of the immune system going crazy.
"This kid... quietly, he even thundered me!"
A bright smile appeared on Professor He's face, "The breakthrough in CAR-T technology, this is a major scientific research achievement! Not bad! Not bad!"
After reading this paper, Professor He hurriedly downloaded the paper and printed it out. Then, Professor He picked up the paper materials, turned around and ran out.
All the way to Fudan Medical College's cancer research room, I found Professor Guo who is engaged in cancer medical technology research.
"Lao Guo, Lao Guo, take a look at this paper."
Professor He shouted and raised the information in Professor Guo.
"paper?"
Professor Guo from the Cancer Research Office looked up at Professor He, "Lao He, you are so excited, does this paper still have some stock?"
"More than stocks?"
When he came to Professor Guo, Professor He handed over the paper, "See it for yourself!"
"CAR-T technology, a preliminary study on the mechanism of shielding T cell information transmission? Author...Lu Li?"
Seeing this title and seeing the author, Professor Guo suddenly became serious, "It turned out to be written by Lu Li. This kid, actually did research on this? It's amazing!"
Picking up the paper, Professor Guo sat down and looked at it seriously.
After reading it over and over again, Professor Guo had a look of surprise on his face, "From the point of view of the experimental procedures and ideas, this new direction of gene editing is...successful?"
"It looks like this!"
Professor He grinned, "I came to you just to repeat this experiment."
"Row!"
Professor Guo quickly got up, "I also want to verify it. If the experiment can be repeated, then...that's awesome!"
A master's degree student actually produced such a highly gold-rich paper, and it was amazing to make such a breakthrough in a cutting-edge scientific research project.
As a result, Professor Guo gave an order to summon all master and doctoral students in the Cancer Research Laboratory, and handed out a paper to them and asked them to repeat Lu Li's experiment.
"Lu Li's paper? CAR-T technology?"
Seeing this paper, the doctoral and master students in the research room were all dumbfounded.
Lu Li, hasn't he been a graduate student for less than a year? This brings out such scientific research results?
Originally, Lu Li's previous "question" made Professor Guo always praise Lu Li, which made everyone feel embarrassed.
Now... what are you doing?
Does this make people live?
Secretly slandering, these "scientific research dogs" can only repeat Lu Li's experiment according to Professor Guo's instructions.
Everyone immediately took action, like an assembly line, continuously preparing sheared proteins, preparing transcriptase, and continuously injecting them into T cells.
Compared to Lu Li alone, completing all the steps by himself, this assembly line model is much more efficient.
In one hour, more than 300 T cell genetic modification specimens have been prepared in the laboratory.
Among the more than 300 specimens, four of them were T cells that realized the mechanism of accurately shielding information transmission.
The experiment was successful!
"Hahahaha! It really succeeded!"
Professor He couldn't help but laugh out loud, "Lu Li really achieved results! Hahahaha!"
The surrounding graduate students and doctoral students were silent.
Professor Guo looked at the successfully prepared T cells, then at Professor He who was smiling from ear to ear, and sighed inwardly.
How could such an outstanding student fall into the hands of Lao He? What a luck!
Later, Professor He published Lu Li's paper and submitted it to the core journal "Chinese Medicine", and attached the record of repeated experiments from Fudan University.
After receiving this submission, the editorial department of "Chinese Medicine" magazine was shocked to see this title, and then saw the attached record of repeated experiments of Fudan University, without saying anything, directly transferred to the reviewer. .
Fudan University has already verified it once, which is clearly a scientific research result that has been tested in practice.
There are several reviewers, some are professors of medicine at Yanjing University, and some are academicians of the Chinese Academy of Sciences.
After receiving the manuscript, the reviewers looked at it seriously.
After reading Lu Li's paper ~EbookFREE.me~ and seeing the repeated test record of Fudan University, the reviewers were amazed again.
The author is a graduate student?
A postgraduate student actually produced such scientific research results?
How strong is Fudan's scientific research strength?
With a sigh, the reviewer arranged for someone to conduct a repeat experiment. The result... once again proved that Lu Li's paper is absolutely correct.
Soon after, the journal "Chinese Medicine" officially published this paper by Lu Li.
Once the thesis came out, it caused a huge shock in the scientific community and also caused a huge response in the society.
"Conquering cancer is no longer a dream. Chinese scientist Lu Li has made a major breakthrough in CAR-T anti-cancer technology!"
Lu Li: Is this a major breakthrough?
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